Crop classification from full-year fully-polarimetric L-band UAVSAR time-series using the Random Forest algorithm

Accurate and timely information on the distribution of crop types is vital to agricultural management, ecosystem services valuation and food security assessment. Synthetic Aperture Radar (SAR) systems have become increasingly popular in the field of crop monitoring and classification. However, the potential of time-series polarimetric SAR data has not been explored extensively, with several open scientific questions (e.g. the optimal combination of image dates for crop classification) that need to be answered. In this research, the usefulness of full year (both 2011 and 2014) L-band fully-polarimetric Uninhabited Aerial Vehicle Synthetic Aperture Radar (UAVSAR) data in crop classification was fully investigated over an agricultural region with a heterogeneous distribution of crop categories. In total, 11 crop classes including tree crops (almond and walnut), forage crops (grass, alfalfa, hay, and clover), a spring crop (winter wheat), and summer crops (corn, sunflower, tomato, and pepper), were discriminated using the Random Forest (RF) algorithm. The SAR input variables included raw linear polarization channels as well as polarimetric parameters derived from Cloude-Pottier (CP) and Freeman-Durden (FD) decompositions. Results showed clearly that the polarimetric parameters yielded much higher classification accuracies than linear polarizations. The combined use of all variables (linear polarizations and polarimetric parameters) produced the maximum overall accuracy of 90.50 % and 84.93 % for 2011 and 2014, respectively, with a significant increase of approximately 8 percentage points compared with linear polarizations alone. The variable importance provided by the RF illustrated that the polarimetric parameters had a far greater influence than linear polarizations, with the CP parameters being much more important than the FD parameters. The most important acquisitions were the images dated during the peak biomass stage (July and August) when the differences in structural characteristics between most crops were the largest. At the same time, the images in spring (April and May) and autumn (October) also contributed to the crop classification since they respectively provided unique information for discriminating fruit crops (almond and walnut) as well as summer crops (corn, sunflower, and tomato). As a result, the combined use of only four acquisitions (dated May, July, August, and October for 2011 and April, June, August, and October for 2014) was adequate to achieve a nearly-optimal overall accuracy. In light of the promising classification accuracies demonstrated in this research, it becomes increasingly viable to provide accurate and up-to-date crops inventories over large areas based solely on multitemporal polarimetric SAR

Developing rAAV production platform with enhanced productivity, scalability and biosafety

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Performance Evaluation of Cluster Validity Indices (CVIs) on Multi/Hyperspectral Remote Sensing Datasets

The number of clusters (i.e., the number of classes) for unsupervised classification has been recognized as an important part of remote sensing image clustering analysis. The number of classes is usually determined by cluster validity indices (CVIs). Although many CVIs have been proposed, few studies have compared and evaluated their effectiveness on remote sensing datasets. In this paper, the performance of 16 representative and commonly-used CVIs was comprehensively tested by applying the fuzzy c-means (FCM) algorithm to cluster nine types of remote sensing datasets, including multispectral (QuickBird, Landsat TM, Landsat ETM+, FLC1, and GaoFen-1) and hyperspectral datasets (Hyperion, HYDICE, ROSIS, and AVIRIS). The preliminary experimental results showed that most CVIs, including the commonly used DBI (Davies-Bouldin index) and XBI (Xie-Beni index), were not suitable for remote sensing images (especially for hyperspectral images) due to significant between-cluster overlaps; the only effective index for both multispectral and hyperspectral data sets was the WSJ index (WSJI). Such important conclusions can serve as a guideline for future remote sensing image clustering applications

Circ_0008234 regulates the biological process of gallbladder carcinoma by targeting the miR-204-5p/FGFR2 axis

Background. Gallbladder carcinoma (GBC) is a common cancer disease with high mortality. Circular RNA_0008234 (circ_0008234) has been shown to play a key role in many tumors, including GBC. However, the function between circ_0008234 and microRNA-204-5p (miR-204-5p) in the progression of GBC has not been clarified. Methods. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expressions of circ_0008234, miR-204-5p and fibroblast growth factor receptor-2 (FGFR2) in GBC cells and tissues. Western blot was used to detect the expression of relative proteins. Cell proliferation, apoptosis, invasion and migration were detected by 3-(4, 5-dimethylthiazol2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, thymidine analog 5-ethynyl-2’-deoxyuridine (EdU) assay, flow cytometry, transwell assay and wound healing assay. Mechanically, the interaction of miR-2045p with circ_0008234/FGFR2 was notarized by dualluciferase reporter assay. A xenotransplantation model was established to study the role of circ_0008234 in vivo. Results. Circ_0008234 and FGFR2 were highly expressed in GBC tissues and cells. Silencing circ_0008234 down-regulated cell proliferation, migration and invasion of NOZ and SGC-996 cells, while miR-204-5p inhibitors reversed these effects. In addition, overexpression of FGFR2 restored the cell malignant behavior of GBC cells inhibited by miR-2045p mimic. Animal experiments confirmed the anti-tumor effect of silenced circ_0008234 in vivo. Conclusion: Circ_0008234 mediated GBC via the miR-204-5p/FGFR2 axis, providing a novel targeted therapy for gallbladder carcinom

Mastering Autonomous Assembly in Fusion Application with Learning-by-doing: a Peg-in-hole Study

Robotic peg-in-hole assembly is an essential task in robotic automation research. Reinforcement learning (RL) combined with deep neural networks (DNNs) lead to extraordinary achievements in this area. However, current RL-based approaches could hardly perform well under the unique environmental and mission requirements of fusion applications. Therefore, we have proposed a new designed RL-based method. Furthermore, unlike other approaches, we focus on innovations in the structure of DNNs instead of the RL model. Data from the RGB camera and force/torque (F/T) sensor as the input are fed into a multi-input branch network, and the best action in the current state is output by the network. All training and experiments are carried out in a realistic environment, and from the experiment result, this multi-sensor fusion approach has been shown to work well in rigid peg-in-hole assembly tasks with 0.1mm precision in uncertain and unstable environments

Brain microvasculature defects and Glut1 deficiency syndrome averted by early repletion of the glucose transporter-1 protein

Haploinsufficiency of the SLC2A1 gene and paucity of its translated product, the glucose transporter-1 (Glut1) protein, disrupt brain function and cause the neurodevelopmental disorder, Glut1 deficiency syndrome (Glut1 DS). There is little to suggest how reduced Glut1 causes cognitive dysfunction and no optimal treatment for Glut1 DS. We used model mice to demonstrate that low Glut1 protein arrests cerebral angiogenesis, resulting in a profound diminution of the brain microvasculature without compromising the blood-brain barrier. Studies to define the temporal requirements for Glut1 reveal that pre-symptomatic, AAV9-mediated repletion of the protein averts brain microvasculature defects and prevents disease, whereas augmenting the protein late, during adulthood, is devoid of benefit. Still, treatment following symptom onset can be effective; Glut1 repletion in early-symptomatic mutants that have experienced sustained periods of low brain glucose nevertheless restores the cerebral microvasculature and ameliorates disease. Timely Glut1 repletion may thus constitute an effective treatment for Glut1 DS

A novel unsupervised Levy flight particle swarm optimization (ULPSO) method for multispectral remote-sensing image classification

The rapid development of earth observation technology has produced large quantities of remote-sensing data. Unsupervised classification (i.e. clustering) of remote-sensing images, an important means to acquire land-use/cover information, has become increasingly in demand due to its simplicity and ease of application. Traditional methods, such as k-means, struggle to solve this NP-hard (Non-deterministic Polynomial hard) image classification problem. Particle swarm optimization (PSO), always achieving better result than k-means, has recently been applied to unsupervised image classification. However, PSO was also found to be easily trapped on local optima. This article proposes a novel unsupervised Levy flight particle swarm optimization (ULPSO) method for image classification with balanced exploitation and exploration capabilities. It benefits from a new searching strategy: the worst particle in the swarm is targeted and its position is updated with Levy flight at each iteration. The effectiveness of the proposed method was tested with three types of remote-sensing imagery (Landsat Thematic Mapper (TM), Flightline C1 (FLC), and QuickBird) that are distinct in terms of spatial and spectral resolution and landscape. Our results showed that ULPSO is able to achieve significantly better and more stable classification results than k-means and the other two intelligent methods based on genetic algorithm (GA) and particle swarm optimization (PSO) over all of the experiments. ULPSO is, therefore, recommended as an effective alternative for unsupervised remote-sensing image classification

A hybrid OSVM-OCNN Method for Crop Classification from Fine Spatial Resolution Remotely Sensed Imagery

Accurate information on crop distribution is of great importance for a range of applications including crop yield estimation, greenhouse gas emission measurement and management policy formulation. Fine spatial resolution (FSR) remotely sensed imagery provides new opportunities for crop mapping at a detailed level. However, crop classification from FSR imagery is known to be challenging due to the great intra-class variability and low inter-class disparity in the data. In this research, a novel hybrid method (OSVM-OCNN) was proposed for crop classification from FSR imagery, which combines a shallow-structured object-based support vector machine (OSVM) with a deep-structured object-based convolutional neural network (OCNN). Unlike pixel-wise classification methods, the OSVM-OCNN method operates on objects as the basic units of analysis and, thus, classifies remotely sensed images at the object level. The proposed OSVM-OCNN harvests the complementary characteristics of the two sub-models, the OSVM with effective extraction of low-level within-object features and the OCNN with capture and utilization of high-level between-object information. By using a rule-based fusion strategy based primarily on the OCNN’s prediction probability, the two sub-models were fused in a concise and effective manner. We investigated the effectiveness of the proposed method over two test sites (i.e., S1 and S2) that have distinctive and heterogeneous patterns of different crops in the Sacramento Valley, California, using FSR Synthetic Aperture Radar (SAR) and FSR multispectral data, respectively. Experimental results illustrated that the new proposed OSVM-OCNN approach increased markedly the classification accuracy for most of crop types in S1 and all crop types in S2, and it consistently achieved the most accurate accuracy in comparison with its two object-based sub-models (OSVM and OCNN) as well as the pixel-wise SVM (PSVM) and CNN (PCNN) methods. Our findings, thus, suggest that the proposed method is as an effective and efficient approach to solve the challenging problem of crop classification using FSR imagery (including from different remotely sensed platforms). More importantly, the OSVM-OCNN method is readily generalisable to other landscape classes and, thus, should provide a general solution to solve the complex FSR image classification problem

Full year crop monitoring and separability assessment with fully-polarimetric L-band UAVSAR:A case study in the Sacramento Valley, California

Spatial and temporal information on plant and soil conditions is needed urgently for monitoring of crop productivity. Remote sensing has been considered as an effective means for crop growth monitoring due to its timely updating and complete coverage. In this paper, we explored the potential of L-band fully-polarimetric Uninhabited Aerial Vehicle Synthetic Aperture Radar (UAVSAR) data for crop monitoring and classification. The study site was located in the Sacramento Valley, in California where the cropping system is relatively diverse. Full season polarimetric signatures, as well as scattering mechanisms, for several crops, including almond, walnut, alfalfa, winter wheat, corn, sunflower, and tomato, were analyzed with linear polarizations (HH, HV, and VV) and polarimetric decomposition (Cloude–Pottier and Freeman–Durden) parameters, respectively. The separability amongst crop types was assessed across a full calendar year based on both linear polarizations and decomposition parameters. The unique structure-related polarimetric signature of each crop was provided by multitemporal UAVSAR data with a fine temporal resolution. Permanent tree crops (almond and walnut) and alfalfa demonstrated stable radar backscattering values across the growing season, whereas winter wheat and summer crops (corn, sunflower, and tomato) presented drastically different patterns, with rapid increase from the emergence stage to the peak biomass stage, followed by a significant decrease during the senescence stage. In general, the polarimetric signature was heterogeneous during June and October, while homogeneous during March-to-May and July-to-August. The scattering mechanisms depend heavily upon crop type and phenological stage. The primary scattering mechanism for tree crops was volume scattering (>40%), while surface scattering (>40%) dominated for alfalfa and winter wheat, although double-bounce scattering (>30%) was notable for alfalfa during March-to-September. Surface scattering was also dominant (>40%) for summer crops across the growing season except for sunflower and tomato during June and corn during July-to-October when volume scattering (>40%) was the primary scattering mechanism. Crops were better discriminated with decomposition parameters than with linear polarizations, and the greatest separability occurred during the peak biomass stage (July-August). All crop types were completely separable from the others when simultaneously using UAVSAR data spanning the whole growing season. The results demonstrate the feasibility of L-band SAR for crop monitoring and classification, without the need for optical data, and should serve as a guideline for future research